![]() Sarasam A, Madihally SV (2005) Characterization of chitosan-polycaprolactone blends for tissue engineering applications. (2015) Morphological, mechanical, and crystallization behavior of polylactide/polycaprolactone blends compatibilized by L-lactide/caprolactone copolymer. ![]() Urbanek O, Sajkiewicz P, Pierini F (2017) The effect of polarity in the electrospinning process on PCL/chitosan nanofibres' structure, properties and efficiency of surface modification. (2019) Effect of extrapallial protein of Mytilus californianus on the process of in vitro biomineralization of chitosan scaffolds. Jaramillo-Martínez S, Vargas-Requena C, Rodríguez-Gónzalez C, et al. (2020) Polycaprolactone/polysaccharide functional composites for low-temperature fused deposition modelling. (2021) Fabrication and characterisation of electrospun Polycaprolactone/Polysuccinimide composite meshes. Voniatis C, Barczikai D, Gyulai G, et al. (2020) Investigation of nanomechanical and morphological properties of silane-modified halloysite clay nanotubes reinforced polycaprolactone bio-composite nanofibers by atomic force microscopy. Li J, Zhuang S (2020) Antibacterial activity of chitosan and its derivatives and their interaction mechanism with bacteria: Current state and perspectives. (2021) Recent advancements in applications of chitosan-based biomaterials for skin tissue engineering. (2021) A systematic review of physical techniques for chitosan degradation. Pandit A, Indurkar A, Deshpande C, et al. (2021) Nanostructure, porosity and tensile strength of PVA/Hydroxyapatite composite nanofiber for bone tissue engineering. Wuriantika MI, Utomo J, Nurhuda M, et al. (2020) Application of injectable silk fibroin/graphene oxide hydrogel combined with bone marrow mesenchymal stem cells in bone tissue engineering. (2021) Self-healable conductive polyurethane with the body temperature‐responsive shape memory for bone tissue engineering. Shaabani A, Sedghi R, Motasadizadeh H, et al. (2020) Composite nanoclay-hydroxyapatite-polymer fiber scaffolds for bone tissue engineering manufactured using pressurized gyration. (2021) Surface modification of a three-dimensional polycaprolactone scaffold by polydopamine, biomineralization, and BMP-2 immobilization for potential bone tissue applications. (2021) Preparation of oriented collagen fiber scaffolds and its application in bone tissue engineering. Prasad A (2021) State of art review on bioabsorbable polymeric scaffolds for bone tissue engineering. (2021) Biomimetic, biodegradable, and osteoinductive Microgels with open porous structure and excellent injectability for construction of microtissues for bone tissue engineering. (2020) Osteogenic effects of the bioactive small molecules and minerals in the scaffold-based bone tissue engineering. Safari B, Aghanejad A, Roshangar L, et al. Su X, Wang T, Guo S (2021) Applications of 3D printed bone tissue engineering scaffolds in the stem cell field. Composite scaffolds of chitosan/polycaprolactone functionalized with protein of Mytilus californiensis for bone tissue regeneration. The scaffolds with the protein added directly presented superior properties in the tests of bioactivity and cellular proliferation, making these composites attractive for the area of bone regeneration.Ĭitation: Miguel-Angel Rojas-Yañez, Claudia-Alejandra Rodríguez-González, Santos-Adriana Martel-Estrada, Laura-Elizabeth Valencia-Gómez, Claudia-Lucia Vargas-Requena, Juan-Francisco Hernández-Paz, María-Concepción Chavarría-Gaytán, Imelda Olivas-Armendáriz. In vitro analysis of biodegradation, bioactivity, and biocompatibility were also performed. The scaffolds were analyzed by Fourier Transformed Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), and Mechanical Compression test to determine the composition, morphology, and mechanical properties of each material. Two methodologies were used for the scaffolds functionalization: (I) an immersion process in a solution with the protein and (II) the protein direct addition during the scaffold synthesis. This study used the extrapalleal fluid protein from Mytilus californiensis because it increases biological processes that support bone regeneration. This research is focused on the evaluation of the properties of Chitosan (Ch)/Polycaprolactone (PCL) scaffolds with the Mytilus californiensis protein by Thermally Induced Phase Separation (TIPS). As a result, the development of bioactive three-dimensional scaffolds for bone regeneration has become a key area of study within tissue engineering. Nowadays, the treatment for bone damage remains a significant challenge.
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